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=== How is the pH of a solution measured? ===
 
=== How is the pH of a solution measured? ===
 
The pH of a solution can be measured by using a [[pH indicator]] like a [[litmus]] paper. A litmus paper dyed blue will change its colour to red when exposed to an acidic solution. Similarly, a litmus paper dyed red will change its colour to blue when exposed to a basic solution. The litmus will be purple when a neutral solution is used. For industrial and laboratory experiments, a [[pH meter]] is used.
 
The pH of a solution can be measured by using a [[pH indicator]] like a [[litmus]] paper. A litmus paper dyed blue will change its colour to red when exposed to an acidic solution. Similarly, a litmus paper dyed red will change its colour to blue when exposed to a basic solution. The litmus will be purple when a neutral solution is used. For industrial and laboratory experiments, a [[pH meter]] is used.
 
=== Why is pH important in biology? ===
 
One reason biologists follow pH is because it can have a tremendous influence on the activity of proteins, including enzymes. Since pH is a measure of the concentration of proteins [H+], it is conceptually a measure of the amount of 'floating charges'. The amino acid histidine readily adds or loses protons onto its side chain in the pH range normal for the blood and body. Thus slight changes in pH can alter the fraction of histidines that are positively charged vs. net neutral. One example where this is the oxygen carrying molecule hemoglobin. In the lungs, the pH is slightly higher than it is in the muscles and tissues; this results in a greater likelihood of certain histidines to be positively charged in the tissues, which in turn drives hemoglobin molecules toward their oxygen <i>releasing</i> state.
 
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